An integrated metabolomic and proteomic approach for the identification of covalent inhibitors of the main protease (Mpro) of SARS-COV-2 from crude natural extractsArticle Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 유전자 메커니즘, 치료제, [키워드] Analysis applied approach baicalein binding carried catalytic characteristic Chinese complementary complex Compound Covalent binder Cys145 database drug target electrophilic Final functional HIS163 HIS164 His41 independent inhibitor inhibitors Ion knowledge M pro M(pro) mass mass spectrometry maturation metabolomic metabolomics Michael molecular dynamics MPro nucleophilic peptide platform polyprotein protease protein-ligand Proteins proteomic proteomics residue SARS-CoV-2 SARS-CoV-2. Scutellaria baicalensis tested the SARS-CoV-2 [DOI] 10.1016/j.talanta.2022.123824 PMC 바로가기
Antibody and T-cell responses by ultra-deep T-cell receptor immunosequencing after COVID-19 vaccination in patients with plasma cell dyscrasiasArticle Published on 2022-11-012022-11-15 Journal: British Journal of Haematology [Category] 진단, [키워드] age anti-CD38 antibodies antibody Antibody Response Antibody testing antibody titres Antigen binding domain BNT162b2 breadth Cell comparable coronavirus disease correlated COVID-19 COVID-19 vaccination demonstrated detectable dose dyscrasia elicit Genome healthy humoral immune immunization Immunocompromised Immunoglobulin Increasing initial Intervention investigated JNJ-78436735 maturation median mRNA vaccine mRNA-1273 Myeloma of BNT162b2 Patient patient population plasma Population proportion receiving Region response spike-specific T-cell Spike-specific T-cell responses Surface glycoprotein T-cell T-cell receptor T-cell Response TCR tested therapy underlying disease vaccination Vaccines Vaccines. [DOI] 10.1111/bjh.18434 PMC 바로가기
The Integral Membrane Protein ZMPSTE24 Protects Cells from SARS-CoV-2 Spike-Mediated Pseudovirus Infection and Syncytia FormationArticle Published on 2022-10-262022-11-15 Journal: mBio [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료제, [키워드] acute respiratory syndrome appear assays catalytic caused Cell cell-cell fusion cell-cell fusion assay cells cellular cellular component cellular membrane contribute coronavirus coronavirus coronavirus SARS-CoV-2 Coronaviruses COVID-19 COVID-19 pandemic dead defend Defense Efficiency ENhance entry of SARS-CoV-2 examined Formation genetic knockout global public health host cell membranes IFITM IFITMs in viral include Infection inhibiting inhibitory activity innate immune innate immune response Integral interact Interaction interfere intrinsic knockdown lentivirus maturation mechanism membrane membrane protein MHV mouse hepatitis virus nuclear overexpression pandemic Plaque assay precursor prelamin A Prevent protease Proteases PROTECT Protective protective role Protein proteolytic proteolytic activity reduce required restriction factor restriction factor. SARS-CoV-2 SARS-CoV-2 pseudovirus infection shown spike Therapeutic benefit transmembrane protein viral infection viral infections virus virus entry viruses wild-type ZMPSTE24 [DOI] 10.1128/mbio.02543-22 PMC 바로가기
Chalcone-amide, a privileged backbone for the design and development of selective SARS-CoV/SARS-CoV-2 papain-like protease inhibitors선택적인 SARS-CoV/SARS-CoV-2 파파인 유사 프로테아제 억제제의 설계 및 개발을 위한 특권적인 백본인 Chalcone-amideReview Published on 2022-10-052022-09-11 Journal: European journal of medicinal chemistry [Category] COVID19(2023년), SARS, 치료제, [키워드] active site acute respiratory syndrome acute respiratory syndrome coronavirus amide antiviral immune responses backbone binding carbon caused chalcone Chalcone amide (N-benzylbenzamide) complex Compound coronavirus COVID-19 COVID-19 pandemic Critical crystal structure effort functional Genetic Host immune responses in some increase Infection inhibitor inhibitors inhibitory potency introduced maturation methyl group Papain Papain-like protease Papain-like protease (PLpro) performed PLPro polyproteins protease Protein Regulation Replication reported Research SAR SARS-CoV SARS-CoV-2 Screening selective shown similarity Structure therapeutic agent therapeutic agents Treatment treatment of COVID-19 understanding unique X-ray X-ray crystal structure. [DOI] 10.1016/j.ejmech.2022.114572 PMC 바로가기 [Article Type] Review
Humoral immunity to SARS-CoV-2 elicited by combination COVID-19 vaccination regimensArticle Published on 2022-10-032022-11-15 Journal: The Journal of Experimental Medicine [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] analysis antibody binding affinity biochemical breadth Cell ChAdOx1 ChAdOx1 vaccine Combination combinations COVID-19 vaccination COVID-19 vaccine effort elicited epitope exhibited Immunity intervals magnitude maturation memory B memory B cell monoclonal antibody mRNA Neutralizing neutralizing antibody regimen regimens response resulting SARS-CoV-2 SARS-CoV-2 pandemic significant difference specificity vaccination Vaccine viral variant while Wuhan-Hu-1 [DOI] 10.1084/jem.20220826 PMC 바로가기
The N501Y Mutation of SARS-CoV-2 Spike Protein Impairs Spindle Assembly in Mouse OocytesArticle Published on 2022-10-012022-11-16 Journal: Reproductive Sciences [Category] COVID19(2023년), SARS, 변종, [키워드] accompanied assembly caused control group COVID-19 pandemic demonstrated female functions Health impairment indicated Infection influence maturation mouse mutant mutant spike protein N501Y N501Y variant Oocyte Oocyte maturation oocytes potential risk Protein provided reproductive SARS-CoV-2 SARS-CoV-2 spike protein significantly increased spike spike protein. Spindle spindle assembly the mean [DOI] 10.1007/s43032-021-00809-7 PMC 바로가기
Structural Basis for the Inhibition of Coronaviral Main Proteases by a Benzothiazole-Based InhibitorArticle Published on 2022-09-182022-11-15 Journal: Viruses [Category] SARS, 변종, 치료제, [키워드] acute respiratory syndrome Analysis Antiviral antivirals Basis binding caused consequence coronavirus Coronavirus-2 Coronaviruses COVID-19 crystal structure defined determinant effective effective inhibitor enzyme Health inhibition inhibitor M pro main protease maturation mechanism MERS-CoV molecular other coronaviruses promote protease provide required SARS-CoV SARS-CoV-2 Spread Treatment unique Vaccine variant viral replication warhead. YH-53 [DOI] 10.3390/v14092075 PMC 바로가기
A key F27I substitution within HCDR1 facilitates the rapid maturation of P2C-1F11-like neutralizing antibodies in a SARS-CoV-2-infected donorArticle Published on 2022-09-132022-11-15 Journal: Cell Reports [Category] SARS, 진단, [키워드] Analysis anti-SARS-CoV-2 antibodies antibody antibody repertoire Bioactivity characterized contribute CP: Immunology deep sequencing developmental Donor early infection elicit event facilitate immunogen information Lineage longitudinal maturation monoclonal NAb neutralizing antibody neutralizing antibody. P2C-1F11 lineage pathway reveal SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2-specific antibody sequence significantly viral infection [DOI] 10.1016/j.celrep.2022.111335 PMC 바로가기
X-ray crystallographic characterization of the SARS-CoV-2 main protease polyprotein cleavage sites essential for viral processing and maturationArticle Published on 2022-09-032022-11-16 Journal: Nature Communications [Category] SARS, 변종, [키워드] Analysis Antiviral binding C-terminal C-terminally capture catalytic cause cleavage cleavage site cleavage sites Complete complex coronavirus 2 COVID-19 enzyme event form Interaction M pro maturation mutant non-structural protein pathogen plasticity polyprotein polyproteins Prevent protease provide representing respiratory responsible SARS-CoV-2 specificity substrates the SARS-CoV-2 therapeutic unique X-ray X-ray crystallographic structure [DOI] 10.1038/s41467-022-32854-4 PMC 바로가기
The Kinetics of SARS-CoV-2 Antibody Development Is Associated with Clearance of RNAemiaArticle Published on 2022-08-302022-11-15 Journal: mBio [Category] COVID19(2023년), SARS, 진단, [키워드] accelerated antibody baseline binding binding activity binding antibody Characteristics clearance COVID-19 COVID-19 disease COVID-19 disease severity Critical death defined development disease disease severity dissemination effective Evolution examined FcγR feature function highlight humoral humoral immune response IgG IgG3 immune immune clearance immune function Immune profile independent Kinetics Longitudinal data longitudinal data modeling maturation monoclonal Mortality natural infection neutralization outcome persistence persistent persistent SARS-CoV-2 plasma viremia plasma predict progression provide SARS-CoV-2 SARS-CoV-2 replication severe COVID-19 Specificities subunit system serology viral clearance viral replication Viremia viremia. [DOI] 10.1128/mbio.01577-22 PMC 바로가기